Biotinylation of interleukin-2 (IL-2) for flow cytometric analysis of IL-2 receptor expression Comparison of different methods
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منابع مشابه
Cellular expression of interleukin 2 (IL-2) and its receptor (IL-2R, CD25) in lung tumours.
In view of the unclear prognostic and diagnostic role of interleukin 2 (IL-2) and its receptor in human tumours, we examined the cellular expression of IL-2 and of the subunit alpha of its receptor (IL-2Ralpha, CD25) in relation to the proliferative activity of various subtypes of lung tumours. The immunocytochemical ABC technique was applied to archival tissue material of neuroendocrine lung t...
متن کاملInterleukin 2 (IL-2) augments transcription of the IL-2 receptor gene.
We demonstrate that purified interleukin 2 (IL-2) can directly upregulate IL-2 receptor expression on phytohemagglutinin-activated T lymphocytes maintained in culture until IL-2 receptor expression had markedly declined. The IL-2-induced increase in IL-2 receptor number is maximal within 12 hr, requires new RNA and protein synthesis, and is mediated by an interaction of ligand with the high-aff...
متن کاملProduction of Interleukin-2 (IL-2) and Expression of IL-2 Receptor in Patients with IgA Nephropathy
BACKGROUND IL-2 production has been measured in several disease including type I diabetes mellitus, systemic lupus erythematosus, acquired immunodeficiency syndrome and active pulmonary sarcoidosis and its pathogenetic role was suggested. In IgA nephropathy, altered T cell subsets were reported to be associated with increased synthesis of IgA. The altered IL-2 production and the expression of I...
متن کاملA COMPARISON OF IL-2 IN NORMAL AND SYMPTOMATIC PULPS
Normal healthy pulpal tissues were obtained from 19 impacted molars and symptomatic samples were obtained from 18 carious molars and premolars clinically diagnosed in all vital pulpal tissues. Student's t-test revealed significant differences in IL-2 concentrations, comparing symptomatic pulpal tissues with normal healthy samples (657, p<O.OI). These results suggest that IL -2 may serve as...
متن کاملPhenotypic Changes Induced by Interleukin-2 (IL-2) and IL-3 in an Immature T-Lymphocytic Leukemia Are Associated With Regulated Expression of IL-2 Receptor
We have previously reported the establishment of an interleukin-3 (IL-3)-dependent and phenotypically myeloid cell line (TALL-103/3), obtained by culturing cells from an immature T-lymphoblastic leukemia in the presence of IL-3. These cells differentiated into a T-lymphoid cell line (TALL-103/2) upon removal of IL-3 and incubation in IL-2. Despite the different phenotype, the two cell lines rem...
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ژورنال
عنوان ژورنال: Journal of Immunological Methods
سال: 1995
ISSN: 0022-1759
DOI: 10.1016/0022-1759(95)00080-t